Characterization of CD8+ virtual memory T cells in IL-4 knockout mice using single-cell RNA sequencing. Characterization of CD8+ virtual memory T cells in IL-4 knockout mice using single-cell RNA sequencing

We profiled the heterogeneity of virtual memory T cells and observed changes in virtual memory T cell subsets under conditions of IL-4 deficiency in mouse spleens using single-cell RNA sequencing. Overall design: Using DEG analysis, we distinguished virtual memory T cells from splenocytes of wild-type B6 mice and IL-4 knock-out mice and compared virtual memory T cells of each condition.