Array comparative genomic hybridization of canine follicular lymphoma (cFL) and canine marginal zone lymphoma (cMZL). Array comparative genomic hybridization of canine follicular lymphoma (cFL) and canine marginal zone lymphoma (cMZL)

The aim of this work was to identify the copy number aberrations (CNAs) by high-resolution array comparative genomic hybridization (aCGH) on 7 dogs with newly diagnosed FL and 5 dogs with newly diagnosed MZL. Overall design: In total, the DNA obtained from 7 cFL and 5 cMZL specimens together with the paired skin biopsies were analyzed using a Agilent-025522 SurePrint G3 Canine CGH Microarray 4x180K (GPL24897). Tumor and reference DNA samples were labelled independently with cyanine 5-deoxyuridine triphosphate (dUTP) and cyanine 3-dUTP, respectively, using SureTag Complete DNA Labeling Kit. Oligo aCGH analysis was performed following the manufacturer’s recommendations, using an Oligo aCGH/ChIP-on-chip Hybridization Kit (Agilent Technologies).