Overview of 32 Th2-specific inducible genes, highlighting their functions and GATA3 dependency.

(*) GATA3 dependency was evaluated by the recovery score (RS) [22].(**) GATA3 dependency was evaluated by gene expression ratio (Gata3/Control siRNA).“Unevaluable” means that mRNA level was too low to detect by qPCR.Unifying 24 genes identified in the present study and previously reported 26 genes [22], 32 genes were defined as Th2-specific inducible genes. Four biological readouts in these 32 genes are shown; (1) changes in gene expression by enforced expression of hGATA3 in STAT6-deficient Th2-1st cells (3rd column), (2) changes in gene expression by GATA3 knockdown in Th2-1st cells (4th column), (3) those in Th2-4th cells (5th column), and (4) those in memory Th2 cells (6th column). In the 3rd column, GATA3 dependency was determined by the recovery score (RS), which is defined as the linear equation below [22]:RS (%) = 100×(C−B)/(A−B).where A indicates the signal intensity of Th2, B indicates the signal intensity of STAT6-deficient Th2, and C indicates the signal intensity of STAT6-deficient Th2 with overexpression of hGATA3. In the 4th, 5th, and 6th column, GATA3 dependency was determined by gene expression ratio (Gata3/Control siRNA). Gene ontology (GO) terms identified by DAVID analysis tool are shown in the 7th column.