Transcriptome profiles of RAW264.7 cells under fibroblast coculture with or without LPS sitimulation and tryptophan metabolites intervention

We performed RNA sequencing to identify the role of fibroblast in the effects of tryptphan metabolites on LPS-stimulated RAW264.7 cells. Overall design: Intestinal fibroblasts were cultured and passaged into 24-well plates. Separately, RAW264.7 cells were passaged and seeded into 0.3 µm pore-size transwell inserts, which were then placed into the 24-well plates containing the fibroblasts. The co-cultures were then exposed to lipopolysaccharide (LPS, 100 µg/mL), indole lactic acid (ILA) or indole acetic acid (IAA) (each at 6 µM), and/or CH223191 (1 µM), depending on the experimental group.