Spatial sequencing of Foreign body granuloma

Although macrophage-epithelioid cell (EPC)-giant cell (GC) differentiation is acknowledged in foreign body reaction (FBR), the exact molecular features remain elusive. To discover the molecular profiles of EPC and GC, we analyzed mouse sponge and silk FBRs by integrating single-cell RNA-sequencing (scRNA-seq) and spatial sequencing. After adjusting tissue permeabilization time to 18 min, we performed spatial sequencing library construction using Visium Spatial Gene Expression Reagent Kits (10x Genomics), according to the manufacturer’s instructions.