ATAC-seq of HFF exposed to salt and heat stress

Primary human foreskin fibroblasts (HFF) were exposed to either salt stress (80mM KCl) or heat stress (44ºC). ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013 HFF were exposed either salt stress (80mM KCl) or heat stress (44ºC) for 1 or 2h. ATAC-seq samples were prepared at the indicated times of infection. Two independent biological replicates were analysed for salt and heat stress and four replicates for untreated cells.