Adipocyte-Specific Ablation Of PU.1 Promotes Energy Expenditure and Ameliorates Metabolic Syndrome In Aging Mice

Here, we investigated the role of adipocyte PU.1 in the development of age-associated metabolic syndrome. We generated mice with adipocyte specific PU.1 knockout, assessed metabolic changes in young and aged PU.1fl/fl (control) and AdipoqCre PU.1fl/fl(aPU.1KO) mice, including body weight, body composition, energy expenditure and glucose homeostasis. We also performed transcriptional analyses using RNA-Sequencing of adipocytes from these mice. Sequencing was performed on adipocyte samples from PU.1 fl/fl (control) and PU.1 fl/fl-AdipoqCre (PU.1 knockout) mice, with 3 replicates in each group.本研究探讨了脂肪细胞PU.1在衰老相关代谢综合征（age-associated metabolic syndrome）发生发展中的作用。我们构建了脂肪细胞特异性PU.1敲除小鼠，对年轻及衰老状态下的PU.1fl/fl（对照）与AdipoqCre PU.1fl/fl（aPU.1KO）小鼠的代谢变化进行了检测，检测指标包括体重、体成分、能量消耗及葡萄糖稳态（glucose homeostasis）。此外，我们采用RNA测序（RNA-Sequencing）对上述小鼠的脂肪细胞开展转录组分析。本次测序的样本取自PU.1fl/fl（对照）及PU.1fl/fl-AdipoqCre（PU.1敲除）小鼠的脂肪细胞，每组设置3个生物学重复。