Comparison of relative mRNA expression for genes encoding sorbitol transporter (SOT), sucrose transporter (SUT), tonoplast monosaccharide transporter (TMT), and vacuolar glucose transporter (vGT) among mature leaves, shoot tips, young fruit (40 DAB) and mature fruit (135 DAB) of apple.

Values are means of three technical replicates of the reverse transcribed RNA sample pooled from 5 biological replicates ± SD.
qRT-PCR was performed with gene-specific primers. For each sample, transcript levels were normalized with those of Actin, and the relative expression levels of each gene were obtained using the ddCT method while expression in mature leaves was designated as ‘1.’