Primary metabolism dataset of Lotus japonicus hemoglobin mutants [Dataset]

Seeds of Lotus japonicus ecotype Gifu B-129 were surface sterilized and seedlings were grown in pots for 7 weeks in a controlled environment chamber, with light intensity of 120-150 umol m-2 s-1, 16 h/8 h light/dark period, and 23ºC /21ºC temperature day/night regime. Non-nodulated plants were supplied with 5 mM ammonium nitrate. Nodulated plants were inoculated with Mesorhizobium loti MAFF303099 and supplied with 0.25 mM ammonium nitrate. Frozen material plant was pulverized in liquid nitrogen and metabolites were extracted following the protocol described by Lisec et al. 2006 (Nature Protocols 1, 387-396). Samples were derivatized with methoxyamine hydrochloride prior to GC-MS analysis. Data were analyzed using Thermo Scientific Xcalibur software. Retention times and mass-to-charge ratio (m/z) were taking into account to identify metabolites against the Golm Metabolome Database.