Gene expression in the murine liver, effect of hepatic expression of Niemann-Pick C1-like 1 protein.

To characterize basal differences in hepatic gene-expression patterns, we performed a microarray analysis using wild-type and L1-Tg mice fed a control fat diet (CE-2: CLEA Japan, Inc., Tokyo, Japan) for two weeks. RNA samples were taken from the livers of mice, and then subjected to a microarray analysis. Comparisons among groups were made by one-color method with normalized data from Cy3 channels for data analysis.