Src modulates its oncogenic signaling via regulation of miRNA-129-3p [microRNA]

To address the molecular mechanisms underlying c-Src-induced cell transformation, we previously developed a model system using Csk-deficient fibroblasts that can be transformed by wild-type c-Src. In this study, we applied this system for the analysis of the potential contribution of mRNA and miRNA to c-Src-induced cell transformation. We found miR-129-3p was downregulated in c-Src-induced cell transformation in a Dox-inducible expression system via c-Src, c-Yes, and Fer. CskKO (Csk-/-) MEFs were transfected with c-Src. Cells were induced c-Src for 0, 6, 24 and 72 hours. Each sample was run in duplicate.