Coronavirus genes were unexpectedly detected in the unidentified clinical samples stored using next-generation sequencing. Viruses

Next-generation sequencing (NGS) is a powerful tool for pathogen identification of clinical samples among well-known microorganism. NGS does not depend on pre-designed primer sequences of pathogen. This may be one of reason for widespread use. In this study, from 2009 to 2016 twenty four of unidentified clinical samples stored were applied to the NGS approach. Human coronavirusOC43 strain genes were unexpectedly detected in almost all samples. This means that coronavirus infection often occurs in various clinical situations. Results also show that coronavirus infection, which is known as common cold, manifests various symptoms as well as symptoms as a common cold.