Enterobacter hormaechei sequencing using MinION. Enterobacter hormaechei

We developed a cgSNP-independent phylogenetic analysis method using ONT read assemblies by focusing on open reading frame (ORF) content patterns, using WGS data of 66 Enterobacter hormaechei strains acquired by both ONT and Illumina sequencing and 162 strains obtained from NCBI database as proof of concept. WGS data were converted to binary sequences based on the presence or absence of ORFs using BLASTn. Phylogenetic trees calculated from binary sequences (ORF trees) were compared with cgSNP trees derived from Illumina sequences. This BioProject is the long-read assemblies with MinION of the three assemblies used in the study: short-read, long-read and hybrid assemblies.