Elucidation of blackening disease in lotus (Nelumbo nucifera Gaertn.) caused by Hirschmanniella diversa Sher. plant metagenome

The lotus (Nelumbo nucifera Gaertn.) is widely cultivated in Asia, but kurokawa-senchu-byo of blackening lotus tuber is serious problem, which is caused by Hirschmanniella diversa Sher of plant-parasitic nematodes. To effectively control the disease, we must elucidate the blackening mechanisms; therefore, we performed soil chemical analysis and evaluation of the disease level in the lotus cultivation fields, identification of chemical components of black spots on the lotus surface, and microbiome analysis of the black spots.