BM-resident BL/6 and ST2-/- ILC2s cultured with or without rmIL-33

To investigate the effect of interleukin (IL-33) on bone marrow (BM) resident type 2 innate lymphoid cells (ILC2s), we treated BL/6 and ST2-knockout (ST2-/-) ILC2s for 24h with recombinant mouse IL-33 We then performed gene expression profiling analysis using data obtained from RNA-seq of 3-4 samples per condition. Overall design: Comparative gene expression profiling analysis of RNA-seq data for FACS-sorted BL/6 ILC2s without IL-33 treatment, BL/6 ILC2s with IL-33 treatment, and ST2-/- ILC2s without IL-33 treatment.