Synchronized HES7 Oscillation In Human Embryonic Stem Cell-Derived Presomitic Mesoderm

Little is known about human segmentation clock during somitogenesis. Human embryonic stem (ES) cell differentiation towards PSM and somite cells provide a window of opportunity to probe for the dynamics of oscillatory gene expression. Using high temporal RNA-seq, we captured a transcriptional signature highly reminiscent of segmentation clock during somite differentiation. Our study provides a novel in vitro system to model human segmentation clock that is otherwise inaccessible during development. Overall design: Human ES differentiation to PSM and somite cell fate, profiled by RNA-seq. Daily profiling, total 12 samples, triplicate for each day of sampling. 30 mins profiling, total 46 samples, replicates for each time points.