Transcriptional profiling of endothelial cells lacking Tgfbr2

Tgfbr2 controls signal transduction after binding activated Tgfb1. Inactivation of this receptor in endothelium results in vascular dysplasia and severe hemorrhage. Here, we define the transcriptional profile of endothelial cells lacking Tgfbr2. Overall design: Endothelial cells were isolated from E14.5 embryonic brains using fluorescent-activated cell sorting. We used the Ai14 allele to induce expression of TdTomato in recombined cells, facilitating cell sorting by detecting TdTomato fluorescence. Total RNA was extracted from 2000 – 5000 cells to synthesize PolyA+ libraries for sequencing in a HiSeq4000 using SE50.