regulation research analysis in of the resuscitation of Mycobacterium marinum

Latent tuberculosis infection (LTBI) affects one third of the human population. The latent Latency has always continued to bebeen a research issue. The mechanism of by which latency is enteredentering latency has been thoroughly studied; however, but not resuscitation has not been well studied. Transcriptional regulation has playedplays an essential role forin the resuscitation mechanism. We applied RNA-seq to explore the resuscitation of Mycobacterium marinum (M. marinum), which is a useful model to study the pathogenesis of Mycobacterium tuberculosis (Mtb). A time course of 18 sequencing profiles of time course were was generated in with six time points (0h、0 h, 0.5h、5 h, 4h、4 h, 12h、12 h, 24h 24 h and 48h)48 h). We systematically described the transcriptome and identified differentlydifferentially expressed genes between adjacent time periods. Furthermore, we found 440 differentlydifferentially expressed genes, and selected the top 10 genes (up-regupregulated and down-regdownregulated) of each periodsperiod to validate byfor validation via qRT-PCR. We also performed WGCNA toonfor all genes and obtained 12 gene modules, in which there are were 6 key gene modules related to time periods of resuscitateresuscitation. Finally, a co-ecoexpression network associated with resuscitate resuscitation was constructed. In general, ourOur study had primarily uncovered M. marinum transcriptional regulation in the recovery process, which could help to gain a better understanding of the regulation system in latentlatency.