Transcriptomics of freezing-thawing in wood frogs

Total body freezing is a highly specialized and rare winter survival strategy that is undertaken by few vertebrates. The freeze-tolerant wood frog can survive freezing of up to 70% of its body water and remain frozen for months. We use RNA-seq data to investigate the gene expression profile in livers of wood frogs from Connecticut, USA as they experience a single freeze-thaw episode. We take a fine time-scale approach and investigate gene expression as frogs enter, exit, and recover from freezing.