Precision Surgery Using Nanosecond Pulsed Electric Field (nsPEF) in Early Hepatocellular Carcinoma: A Phase I Clinical Trial Integrating Multi-Omics Analysis

This dataset comprises the raw microbiome sequencing data generated from a Phase I clinical trial (NCT04309747) investigating Nanosecond Pulsed Electric Field (nsPEF) as a non-thermal ablation therapy for early-stage Hepatocellular Carcinoma (HCC). The data captures longitudinal changes in the oral and gut microbiota of HCC patients undergoing nsPEF treatment. Analysis of this data, as presented in the associated publication, revealed that:
Pre-operative microbial profiles were enriched with opportunistic pathogens.
Post-operative shifts showed a significant increase in beneficial, short-chain fatty acid-producing bacteria following nsPEF ablation.
Baseline microbiome composition was predictive of treatment outcome, enabling the construction of a high-performance prognostic model.
By providing comprehensive microbiome data linked to a novel ablation therapy and detailed clinical outcomes, this dataset serves as a valuable resource for:
Investigating the systemic biological effects of local tumor ablation.
Exploring the role of the microbiome in cancer therapy response.
Developing microbial biomarkers for personalized cancer treatment strategies.
Enabling meta-analyses and validation studies in the field of oncomicrobiology.