Data from: Miniaturized optically-generated Bessel beam ultrasound for volumetric transcranial brain stimulation

Non-invasive stimulation of small, variably shaped brain sub-regions is
crucial for advancing our understanding of brain functions. Current
ultrasound neuromodulation faces two significant trade-offs when targeting
brain sub-regions: miniaturization versus volumetric control, and spatial
resolution versus transcranial capability. Here, we present an
optically-generated Bessel beam ultrasound (OBUS) device designed to
overcome these limitations. This miniaturized device, measuring 2.33 mm in
diameter, delivers a column-shaped field achieving a lateral resolution of
152 μm and an axial resolution of 1.93 mm, enabling targeting of brain
sub-regions with an elongated volume of tissue activation.
Immunofluorescence imaging of mouse brain slices confirms its ability to
stimulate cells at depths up to 2.1 mm. Additionally, OBUS outperforms
conventional Gaussian ultrasound in transcranial transmission efficiency
and beam shape preservation. Electrophysiological recordings and
functional MRI captured rodent brain responses evoked by OBUS,
demonstrating OBUS’s ability to non-invasively activate neural circuits in
intact brains. This technology offers new possibilities for studying brain
functions with precision and volumetric control.