AP-induced hyperphagia is associated with a unique hypothalamic gene expression signature that is abrogated by adjuvant drug treatment

Antipsychotic-induced hyperphagia is associated with a unique hypothalamic gene expression signature that is abrogated by adjuvant drug treatment. C57BL/6 female mice were purchased from Jackson labs at 9 weeks of age. All mice were fed normal chow until 10 weeks of age. Olanzapine was compounded into high fat diet (HFD) (45 kcal% from fat) at a concentration of 54 mg/kg of diet (OLZ-HFD, Research Diets, Inc., D09092903, New Brunswick, NJ). Control animals were fed HFD (45 kcal % from fat D09092903) . Minocycline (MINO) was administered in the drinking water at a dose of 0.6mg/ml. At 10 weeks of age mice were randomly divided into four treatment groups (control, OLZ, MINO and OLZ + MINO) and fed these diet/water combinations for the 12 week duration of the study. Mice were then sacrificed and hypothalami dissected for RNA seq analyses.