Supplemental Material for Nelson and Ambros, 2021

Figure S1 contains sanger sequencing confirming <i>let-7 </i>microRNA sequence in <i>C. macrosperma</i>. Figure S2 contains an expanded view of the <i>C. sulstoni </i>and <i>C. macrosperma </i>genomic regions that are syntenic to the <i>let-7 </i>genomic region in <i>C. elegans. </i>Figure S3 contains the <i>C. afra </i>genomic regions that are syntenic to the <i>let-7 </i>genomic region in <i>C. elegans. </i>Figure S4 contains the developmental growth rates of <i>C. elegans</i>, <i>C. macrosperma, </i>and <i>C. sulstoni. </i>Figure S5 contains <i>lin-4 </i>microRNA expression throughout development in <i>C. elegans</i>, <i>C. macrosperma, </i>and <i>C. sulstoni</i>. Figure S6 contains a <i>lin-41 </i>gene tree in <i>Caenorhabditis </i>species. Figure S7 contains examples of the integration of <i>lin-4 </i>and <i>let-7-</i>family<i> </i>microRNAs in <i>C. elegans </i>and <i>C. sulstoni</i>. Figure S8 contains the predicted minimum free energy hybridizations of <i>let-7-</i>family microRNAs with complementary sites in the lin-41 mRNA 3' UTR in <i>Caenorhabditis </i>species. Table S1 contains <i>let-7-</i>family microRNA sequences in <i>Caenorhabditis </i>species. Table S2 contains homologs of heterochronic genes in <i>C. elegans </i>and <i>C. sulstoni</i>. Table S3 contains lin-41 3' UTR bulge-SM+SUP information in <i>Caenorhabditis </i>species. Table S4 contains lin-41 3' UTR GU-SM+SUP information in <i>Caenorhabditis </i>species. Table S5 contains a list of <i>Caenorhabditis </i>strains used in this study. Table S6 contains a list of oligos used in this study. Table S7 contains information on the <i>C. sulstoni</i> <i>lin-41::GFP </i>allele generated in this study. Table S8 contains the normalized reads used for Figure 3. Table S9 contains the exact timing of larval molts used for Figure S4.