Transcriptomic analysis-based study on the response mechanism of juvenile golden cuttlefish (Sepia esculenta) to benzo[a]pyrene exposure

Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon from incomplete combustion, builds up in coastal regions through river runoff, atmospheric deposition, and coastal activities. BaP's lipophilicity and stability lead to persistent environmental impacts due to its resistance to degradation. The economically valuable golden cuttlefish, Sepia esculenta, often spawns and hatches in shallow waters, making it prone to BaP exposure. This study employs transcriptomic analysis to initially investigate juvenile golden cuttlefish's response to BaP. The results indicate that BaP exposure significantly affects various physiological and molecular functions of the juveniles, especially highlighting the link between BaP exposure and enhanced immune and inflammatory responses, disturbances in metabolism and growth development, as well as impairments in nervous system functions. Functional enrichment and PPI network analyses identified key genes such as HSGALT-like, ASAH1-like, and GTL-like in the BaP response. These metabolic genes showed a suppression trend within five days of exposure, affecting lipid metabolism, energy transformation, and digestion. The study offers novel insights into BaP's effects on juvenile golden cuttlefish and marine life, aiding marine ecosystem and biodiversity conservation.