Figure 4: Mice’s exploration of the Elevated Plus Maze (EPM) following Dominant-Submissive Relationship (DSR) testing.

Reiteration of the EPM after DSR testing enabled the comparison of Dom and Sub mice’s risk assessment to that at baseline. Sub mice demonstrated increased exploration of the open portions of the maze after repeated social defeat, measured by the portion of time spent in open arms (A) and the portion of total arm entries attributed to the open arms (B). Both mice strains displayed markedly reduced locomotion in the second iteration of EPM testing (C). Data are presented as mean ±SEM, n=10; variables contributing to statistical significance by repeated-measures two-way ANOVA indicated as: strain (##-p<0.01, ###-p<0.001), DSR testing (+-p<0.05, ++++-p<0.0001) and strain-DSR interaction (^^^-p<0.001); OT/TT: time in open arms/total time; OE/TE: entries to open arms/total arm entries.

在完成DSR测试后的EPM重复测试中，Dom和Sub小鼠的风险评估与基线值进行了比较。Sub小鼠在经历反复的社会挫败后，对迷宫开放区域的探索行为增加，这一行为通过在开放臂中度过的时间比例（A）以及归因于开放臂的总臂进入次数比例（B）进行测量。两种小鼠品系在EPM测试的第二轮中均表现出明显的运动能力下降（C）。数据以平均值±标准误（SEM）的形式呈现，样本量n=10；通过重复测量的双因素方差分析显示统计学上显著的变量包括：品系（##-p<0.01，###-p<0.001）、DSR测试（+-p<0.05，++++-p<0.0001）以及品系-DSR的交互作用（^^^-p<0.001）；OT/TT：开放臂中的时间/总时间；OE/TE：进入开放臂的次数/总臂进入次数。