Tau accumulation induces microglial state alterations in Alzheimer’s disease model mice (snRNA-seq, part 1)

Unique microglial states have been identified in Alzheimer’s disease (AD) model mice and postmortem AD brains. Although it has been well documented that amyloid-β accumulation induces the alteration of microglial states, the relationship between tau pathology and microglial states remains incompletely understood because of a lack of suitable AD models. In the present study, we generated a novel AD model mouse by the intracerebral administration of tau purified from human brains with primary age-related tauopathy into AppNL-G-F/MAPT double knock-in (dKI) mice. DAPI positive cell nuclei were isolated from the hippocampi of the double knock-in model mice with or without tau administration and analyzed using single-nucleus RNA sequencing (snRNA-seq).