Data from: Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with
2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38–46),
Dicerca aenea validiuscula Semenov, 1895 – 2n=20 (18+Xyp) and
Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 – 2n=16 (14+Xyp)
were studied using conventional staining and different chromosome banding
techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin
A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly
visible in all four species which indicates a small amount of constitutive
heterochromatin (CH). There were no signals after DAPI staining and some
positive signals were discovered using CMA3 staining demonstrating absence
of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus
organizing regions (NORs) were revealed using Ag-NOR technique;
argentophilic material mostly coincides with positive signals obtained
using CMA3 staining.