Next Generation Sequencing to compare gene expression of PC-3M-Pro4 in cell culture and in zebrafish metastasis

We report how engrafted human prostate cancer cells respond to zebrafish microenvironment during metasatic colonization. Overall design: RNA was isolated from metastasese in zebrafish (70 fish/group), from uninjected fish (70fish/group), and from cells in culture, in triplicate. Transcriptomics of human cancer cells were compared between in culture and in metastases. PBS-injected fish was set as control to analyze how engrafted cancer cells modulate gene expression of the host stromal cells