soil metagenome

Rhizosphere soil samples were collected in replicates from each treatment from greenhouse and field experiments. Briefly, 5 plants per replication from each treatment were uprooted and bulk soil was removed by shaking the plants. The soil attached to the roots were collected as rhizosphere soil samples, five cores of soil samples from each replication were thoroughly mixed to make one composite sample, and three samples were collected from each treatment. In order to minimize the error, three cores of DNA were extracted from each sample, mixed thoroughly in order to make one sample.