Pyrus x bretschneideri strain:Chinese White Pear Transcriptome or Gene expression. Pyrus x bretschneideri strain:Chinese White Pear

Ten different tissues of the pear plant (Pyrus bretschneideri Rehd. cv. Dangshansuli), including tender shoots, young leaves, extending leaves, mature leaves, flowers and fruit samples (also containing immature seeds) of five different developmental stages were collected and used for RNA preparation and cDNA library construction. Using high-throughput Illumina RNA-seq technology, De novo transcriptome assembly of P. bretschneideri Rehd. was performed at an unprecedented depth (5.47 gigabase pairs). Approximate 60.77 million reads were obtained, trimmed, and assembled into 90,227 unigenes, with an average length of 508 bp and an N50 of 635 bp, which consisted of 17,619 contig clusters and 72,608 singletons. Out of the 90,227 unigenes, 25,415 unigenes (28.17%) were larger than 500 bp in length and 8,452 (9.37%) were larger than 1,000 bp. Sequence similarity analyses against six public databases (Uniprot, NR and COGs at NCBI, Pfam, InterPro and KEGG) found 61,636 unigenes that could be annotated with gene descriptions, conserved protein domains, or gene ontology terms. 34.60% of unigenes (31,215) were annotated against KEGG in 121 known metabolic or signalling pathways.