Data for: Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease

Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease. Tissue engineered blood vessels (TEBVs) hold great promise for clinical use in patients with end stage renal disease (ESRD) requiring vascular access for hemodialysis. Our group has previously developed a way to generate TEBVs in situ, by utilizing foreign body response to polymeric rods that are implanted subcutaneously (Rothuizen TC, Rotmans JI et.al, Biomaterials. 2016 Jan). In the present study, we aimed to investigate the origin of the cells in the tissue capsules (TCs) that are formed around the implanted rods. In addition, we aimed to study the effect of chronic kidney disease (CKD) on TC formation. For this purpose, we utilized a rat model of CKD, which we combined with a BM-transplantation using GFP-labeled cells. These experiments revealed that both bone-marrow derived- as well as tissue resident inflammatory cells contribute to TC formation. In addition, we show that macrophages serve as precursors of myofibroblasts in mature TCs. The presence of CKD did not significantly alter the process of TC formation, which holds the potential to support our approach for future clinical use in hemodialysis patients. The raw data files contain data on each experimental animal for immunohistochemical analysis, RNA expression and laboratory measurements (blood urine nitrogen, creatinine, %of GFP+ bone marrow derived cells) of experimental model. In addition, files include data on statistical tests used in this work such as column statistics and Mann-Whitney test. All data is presented as text documents and was transferred from GraphPad Prism 7.03 program which was used for data analysis.慢性肾脏病大鼠模型中骨髓来源细胞对原位工程化组织囊的贡献。组织工程血管（TEBVs）在需要血液透析血管通路的终末期肾病（ESRD）患者的临床应用中具有广阔前景。本课题组此前已开发出一种原位制备组织工程血管的方法，该方法通过皮下植入聚合物棒引发的异物反应实现（Rothuizen TC、Rotmans JI等，《生物材料》，2016年1月）。本研究旨在探究植入聚合物棒周围形成的组织囊（TCs）内细胞的来源，同时分析慢性肾脏病（CKD）对组织囊形成过程的影响。为此，我们构建了慢性肾脏病大鼠模型，并结合绿色荧光蛋白（GFP）标记细胞的骨髓移植（BM-transplantation）技术。实验结果表明，骨髓来源细胞与组织驻留炎症细胞均参与组织囊的形成。此外，本研究证实，成熟组织囊中巨噬细胞可作为肌成纤维细胞的前体细胞。慢性肾脏病的存在并未显著改变组织囊的形成过程，这为该方法未来应用于血液透析患者的临床治疗提供了可行性。本研究的原始数据文件涵盖所有实验动物的相关数据，包括免疫组织化学分析、RNA表达检测以及实验模型的实验室检测指标（血尿素氮、肌酐、GFP阳性骨髓来源细胞占比）。此外，数据文件还包含本研究中使用的统计检验方法相关数据，如列统计量与曼-惠特尼检验（Mann-Whitney test）。所有数据均以文本文件形式存储，来源于用于数据分析的GraphPad Prism 7.03软件。