Sample summary and analysis of biopsy samples collected during the New Zealand Australian Antarctic Ecosystems Voyage 2015

Biopsy samples were collected from humpback (n=10) and blue whales (n=1) during the NZ/Aus Antarctic Ecosystems Voyage 2015. Biopsy collection from humpback and blue whales was attempted from the bow of the ship using Larsen rifles. Biopsying blue whales from the bow of the RV Tangaroa proved challenging due to the ship’s manoeuvrability and the limited capacity to change speed rapidly. Biopsy samples were split between All Protect (Qiagen), 70% ethanol, and freezing at -20C. .This dataset consists of an excel spreadsheet (biopsy_events.xlsx) summarising biopsy events containing the fields:Date_taken (in UTC)Location (general) where sample collected LatitudeLongitudeIndividual IDSample IDName of samplerSample typePreservative usedSpecies sampledAn excel spreadsheet (Biopsy sample info datasheet AEV 2015.xlsx) details the biopsy processing that occurred upon collection of a sample. Where possible, each sample was split and preserved in 2 x All Protect, 1 x EtOH and 1 x -80 degrees Celsius. Samples preserved in All Protect and 70% ethanol are stored at the Australian Antarctic Division and samples preserved at -80C are stored at NIWA Wellington. A subsample of the Antarctic blue whale biopsy wassubmitted to the IWC-recognised genetic repository for Antarctic blue whale at NOAA Southwest Fisheries, La Jolla. Biopsy samples were processed to determine sex and the results are held in: TAN1502_Whale biopsy samples.xls

本数据集的活检组织样本采集自2015年新西兰/澳大利亚南极生态考察航次期间获取的座头鲸（n=10）与蓝鲸（n=1）。
研究团队使用拉森步枪（Larsen rifles）从考察船船艏尝试采集座头鲸与蓝鲸的活检样本。但尝试从“唐加罗阿号”科考船（RV Tangaroa）船艏采集蓝鲸活检样本时，受限于该船的操纵性能与快速调速能力不足，该操作颇具挑战性。
活检样本被分别分装至All Protect（Qiagen）组织保存液、70%乙醇以及-20℃冷冻三种条件下保存。
本数据集包含一份Excel表格（biopsy_events.xlsx），汇总了所有活检事件的相关信息，字段包括：采样日期（UTC时间）、概略采样地点、纬度、经度、个体编号、样本编号、采样人员姓名、样本类型、所用保存剂、采样物种。
另有一份Excel表格（Biopsy sample info datasheet AEV 2015.xlsx）详细记录了样本采集后的处理流程。在条件允许的情况下，每份样本均被分装为：2份All Protect保存液、1份70%乙醇以及1份-80℃冷冻保存。
采用All Protect与70%乙醇保存的样本现存放于澳大利亚南极署（Australian Antarctic Division），-80℃冷冻保存的样本则存放于新西兰国家水与大气研究所惠灵顿分部（NIWA Wellington）。
本次采集的南极蓝鲸活检子样本已提交至国际捕鲸委员会（IWC）认可的南极蓝鲸遗传资源库，该库位于美国国家海洋和大气管理局（NOAA）西南渔业科学中心拉霍亚分部。
研究人员对活检样本开展了性别鉴定相关实验，相关结果存储于文件TAN1502_Whale biopsy samples.xls中。