Data from: Genetic Architecture of Resistance to Stripe Rust in a Global Winter Wheat Germplasm Collection

Virulence shifts in populations of Puccinia striiformis f. sp. tritici (Pst), the causal pathogen of wheat stripe rust, are a major challenge to resistance breeding. The majority of known resistance genes are already ineffective against current races of Pst, necessitating the identification and introgression of new sources of resistance. Germplasm core collections that reflect the range of genetic and phenotypic diversity of crop species are ideal platforms for examining the genetic architecture of complex traits such as resistance to stripe rust. We report the results of genetic characterization and genome-wide association analysis (GWAS) for resistance to stripe rust in a core subset of 1175 accessions in the National Small Grains Collection (NSGC) winter wheat germplasm collection, based on genotyping with the wheat 9K single nucleotide polymorphism (SNP) iSelect assay and phenotyping of seedling and adult plants under natural disease epidemics in four environments. High correlations among the field data translated into high heritability values within and across locations. Population structure was evident when accessions were grouped by stripe rust reaction. GWAS identified 127 resistance loci that were effective across at least two environments, including 20 with significant genome-wide adjusted P-values. Based on relative map positions of previously reported genes and QTL, five of the QTL with significant genome-wide adjusted P-values in this study represent potentially new loci. This study provides an overview of the diversity of Pst resistance in the NSGC winter wheat germplasm core collection, which can be exploited for diversification of stripe rust resistance in breeding programs. Resources in this dataset:Resource Title: SUPPLEMENTAL MATERIAL FOR BULLI, ET AL, 2016. File Name: Web Page, url: http://www.g3journal.org/content/suppl/2016/05/25/g3.116.028407.DC1 Files in this Data Supplement:

Table S2 - Pearson correlation coefficients among the best linear unbiased estimates (BLUEs) of infection type (IT) and disease severity (SEV) for individual locations (MTV and PLM) and across locations (ALL). (.pdf, 257 KB)
Table S1 - Predominant races of Puccinia striiformis f. sp. tritici during the 2011 to 2014 crop seasons. (.pdf, 336 KB)
Table S3 - Loci associated with resistance to Puccinia striiformis f. sp. tritici in the global winter wheat germplasm collection in at least two environments (with marker-wise P

小麦条锈病病原菌 Puccinia striiformis f. sp. tritici (Pst) 种群中的致病力变异，对小麦条锈病抗性育种构成了重大挑战。已知的大多数抗性基因对当前的 Pst 菌株已失去效力，迫切需要识别和导入新的抗性来源。反映作物物种遗传和表型多样性的种质核心集合是研究复杂性状（如抗条锈病）遗传结构理想的平台。本研究基于对国家小麦种质资源库（NSGC）中 1175 个样本的基因分型（使用小麦 9K 单核苷酸多态性（SNP）iSelect 测序）和四个环境中的幼苗及成年植株在自然病害流行条件下的表型鉴定，对条锈病抗性进行了遗传特征描述和全基因组关联分析（GWAS）。田间数据间的高度相关性转化为各地点及地点间的高遗传力值。当样本根据条锈病反应进行分组时，呈现出明显的种群结构。GWAS 共鉴定出 127 个在至少两个环境中有效的抗性位点，其中 20 个位点的全基因组调整 P 值具有统计学意义。基于先前报道的基因和 QTL 的相对图谱位置，本研究中五个具有显著全基因组调整 P 值的 QTL 可能代表新的位点。本研究概述了 NSGC 冬小麦种质核心集合中 Pst 抗性的多样性，这些资源可被用于育种计划中条锈病抗性的多样化。本数据集中包含的资源：

资源标题：BULLI 等人，2016 年补充材料。
文件名：网页，URL：http://www.g3journal.org/content/suppl/2016/05/25/g3.116.028407.DC1
文件列表：
表 S2 - 个体地点（MTV 和 PLM）和地点间（ALL）感染类型（IT）和病害严重度（SEV）最佳线性无偏估计（BLUE）的皮尔逊相关系数（.pdf，257 KB）
表 S1 - 2011 至 2014 年作物季节中 Puccinia striiformis f. sp. tritici 的主要菌系（.pdf，336 KB）
表 S3 - 在至少两个环境中与 Puccinia striiformis f. sp. tritici 抗性相关的位点（以标记为基础的 P 值）