RRL of a trapdoors spiders (family Nemesidae) 454 Raw sequence reads

The goal of this study was developed a bioinformatic tool for discovering and selection molecular markers using NGS data.These markers will be applied for phylogenetics, population genetics and phylogeography studies.We sequenced (454 GS-FLX Titanium) intra- and interspecific pooled data from three individuals of the Nemesia genus (two individual of N. raripilia and one individual of Nemesia sp.) and one of the Iberesia genus (Iberesia brauni).RRL was prepared using the enzyme NotI and the size selection of the DNA fragments was performed by excision from an electrophoretic gel.