Transcriptional profile of EHEC O157 TUV93-0 overexpressing PsrA or PsrB
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17798
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PsrA and PsrB were found to repress Type III secretion (T3S) when provided in multicopy. Total RNA was extracted at late exponential phase in T3S permissive media (MEM-HEPES). Transcriptional profiles were expressed relative to the vector control (pGEM). Dual colour design. Cy5 labelled cDNA from TUV93-0 pPsrA or pPsrB cultures were hybridised against TUV93-0 pGEM Cy3 labelled cDNA. Data were normalised using the Genespring GX 7.3.1 analysis package.
创建时间:
2012-03-21



