Next generation sequencing contributes to the quantitative analysis of AGEs stimulated HK-2 cell transcriptome

We sequenced the HK-2 gene expression profile stimulated by ages by high-throughput RNA analysis. Select Ctrl group and ages group, Differentially expressed genes (DEGs) were analyzed. Under the screening condition of fold change greater than or equal to 2, P < 0.05. We obtained 284 up-regulated genes and 193 down-regulated genes. It can be seen that there are obvious gene differences between Ctrl group and ages group (Fig. 3a). Gene ontology analysis (go) was completed. Through goseq software, the biological process of quantitative distribution of rich differential genes was intuitively reflected (BP), molecular function (MF) and cell composition (CC). In addition, in order to explore the impact of ages on cell pathways, we used the Kyoto Encyclopedia of biology to analyze the significantly rich metabolic pathways. There are 221 differentially expressed genes in Ctrl group and ages group. A total of 276 pathways have been established, of which 46 pathways have p < 0.05, and the first 20 pathways are shown in Figure 2C. shCtrl-Normal group，shCtrl-AGEs group，shJMJD1A-AGE group