The comparison between the simulation and experimental results of five signaling molecules under activation of EGF and inhibition of TSC2.

The P-values were calculated by Wilcoxon test and used to test the changes in simulation results after perturbation. The upward arrow (↑) indicates that the perturbation caused a rise in the proportion of molecules in active form; the straight line (-) indicates no change, and the downward arrow (↓) indicates decrease in the proportion of molecules in active form.