Increased RNA Polymerase Activity at CTCF binding sites on the Epstein Barr Virus Genome During Reactivation from Latency

We used Precision Nuclear Run-on followed by Deep Sequencing (PRO-Seq) to investigate RNA Polymerase (Pol) activity during Epstein-Barr Virus (EBV) reactivation in EBV positive Burkitt's lymphoma cell lines Mutu-I and Akata. Nuclei were harvested from latent cells and after treatment with NaB/TPA (Mutu-I) or anti-IgG (akata) to stimulate reactivation at 1 and 4 and 12h. We identified multiple sites on the EBV genome enriched with Pol displaying distinct patterns of activity, which showed an association with CTCF and open chromatin. PRO-Seq data from two experiments. Latent Mutu-I cells and 1, 4 and 12 h post-treatment with NaB/TPA (2/3 biological replicates per time point). Latent Akata cells and 1, 4 and 12 h post-treatment with anti-IgG (3 biological replicates per time point). All samples contain Drosophila spike-in.