Single-cell RNA sequencing reveals a role of Phf2 in promoting the exit of postnatal neural stem cell from shallow quiescence.

Dentate gyrus single cell mRNA profiles of 30-day-old wild-type (WT) and Phf2 conditional knockout (Phf2−/−) mice were generated by single cell sequencing. We performed data filtering: i) for the genes expressed in at least 30 cells and cells with detected expression of at least 200 genes and no more than 8000 genes; ii) cells with the percentage of expressed mitochondrial genes no more than 10%. Potential cell doublets were filtered out using scrublet (https://pypi.org/project/scrublet/). Single cell clustering was done using scanpy package (https://scanpy.readthedocs.io/en/stable/). Single cell mRNA profiles of 30-day old Nestin-Cre Tg wild type (Phf2+/+) control mice and conditional KO (Nestin-Cre Tg, Phf2flox/flox) mutant mice.