Quantification of the colocalization of Gar1M1-mCherry or Gar1-mCherry with Fbl-eGFP and of Nhp2-RFP or Nhp2P83A-RFP with Fbl-eGFP from Fig 2 shown with Pearson’s coefficient.

Significance was determined using a t test (p < 0.05) with N = 30 (Gar1) and 26 (Gar1M1) for the Gar1 set and N = 24 (Nhp2) and 21 (Nhp2P83A) for the Nhp2 set. These experiments were repeated with nucleoli from the oocytes of 3 different frogs and were significant each time (Supplementary data file: S1_Data.xlsx).
(XLSX)