Long noncoding RNA LNMAT1 promotes lymphatic metastasis in bladder cancer

Long non-coding RNAs (lncRNAs) are a large class of non-protein-coding transcripts that are over 200nt in length. LncRNAs have emerged as key regulator of biological process involved in the development and progression of bladder cancer. Bladder cancer is one of the most common genitourinary malignancies worldwide, with approximately 429,800 new cases and 165,100 deaths annually in the world. To identify critical lncRNAs that contributes to the progression of bladder cancer, Next generation sequencing (NSG) was performed in five paired high-grade muscle invasive bladder cancer (MIBC) and normal adjacent tissues (NAT), and in five lymph node (LN)-positive and LN-negative bladder cancer tissues. Our results indicate that the differential expressed lncRNAs in both MIBC tissues and LN-positive tissues associated in a variety of biological functions, such as metastasis. To identify critical lncRNAs that contributes to the progression of bladder cancer, NSG was performed in five paired high-grade muscle invasive bladder cancer and normal adjacent tissues and in five lymph node-positive and lymph node-negative bladder cancer tissues. The RNA of tissue was extracted and hybridized on illumina Hiseq 2500.