Ebraccatus_Priority_Effects

The EPE was a collection of short term studies in which the eggs of hourglass treefrogs were inoculated with bacteria in different orders in order to test for the presence of priority effects in tadpole bacteria community development. The first experiment was an inoculation experiment with frog embryos using two bacterial isolates. To complement our tadpole experiment, a follow-up experiment was conducted in which broth containing wells within a well-plate were similarly inoculated with bacteria under two nutrient concentrations (Full LB broth or a 1:20 dilution LB broth). We then conducted a third test in which embryos were inoculated first with a single bacterium and then with a synthetic community of bacteria.The sequences included are 16S rRNA sequences of the V4 variable region.