Supplementary tables. Supplementary material for ‘A simple dialysis device for large DNA molecules’

The potential of
genomic DNA is realized when new modalities are invented that manipulate
large DNAs with minimal breakage or loss of sample. Here, we describe a
polydimethylsiloxane–polycarbonate membrane device to remove small molecules
from a sample while retaining large DNAs. Dialysis rates dramatically change
as DNA size in kb (M) increases and DNA dimensions become comparable to pore
size, and chain characteristics go from rod-like to Gaussian. Consequently,
we describe empirical rates of dialysis, R, as a function of M as falling
into two regimes: DNAs ≤ 1 kb show R(M) ∼e-t/τM
(t = time, τM = time constant), while DNAs ≥1.65 kb slowly passage with R(M) ∼M-1.68;
such partitioning potentiates single-molecule imaging.