Gene expression of central amygdala of mice

Purpose: The goals of this study are to screen out the changes of gene expression profile in response to high fat diet, an mRNA-seq was performed in central amygdala Methods: 6-week-old male wild-type mice were fed with high fat diet (HFD, containing 45% fat) or normal chow diet (ND) for 5 months. And then their central amygdala tissues were removed and total RNA was extracted to perform RNA sequencing. Results:The KEGG pathway analysis showed that genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after HFD Conclusions: Our study represents the first detailed analysis of central amygdalar transcriptomes of mice subjected to high fat diet, with biologic replicates, generated by RNA-seq technology. Our results show genes related to neuroactive ligand-receptor interaction and calcium signaling pathway were dramatically upregulated after high fat diet feeding,which hint at the importance of ion channel in central amygdala after high fat feeding. Cental amygdalar mRNA profiles of mice challenged with 20-week normal chow diet or high fat diet