PfJ2 co-immunoprecipitating proteins identified by tandem mass spectroscopy.

PfJ2 was immunoprecipitated from PfJ2apt parasite lysates using anti-HA antibodies, and co-immunoprecipitating proteins were identified by tandem mass spectrometry (MS/MS) analysis. Control parental parasites (lacking HA-tagged PfJ2) were also used for immunoprecipitation and analyzed in the same manner. Abundance of each identified protein was calculated by summing the total MS1 intensities (highlighted in yellow) of all matched peptides for each selected protein, and normalizing by the total summed intensity of all matched peptides in the sample. (XLSX)