Wirasinha.migec.txt.zip

The file contains mouse T cell receptor (TCR) sequences collected by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Except for the header row, each row contains information about a unique TCR nucleotide sequence. Columns 1-11 contain output from MIGEC software. Columns 12-16 contain information about sample origin, detailed as follows: column 12 "sample_id" is an identifier for the sample of origin; column 13 "tcr_b" specifies the TCR beta chain status of donor mouse ("poly" = C57BL/6 inbred mouse strain); column 14 "organ" specifies organ of origin (thymus, "gut" = small intestine and spleen); column 15 "subset" specifies the T cell subset of origin ("t_p" = pre-selection thymocytes; "t_1" = wave 1 thymocytes; "t_r" = thymic T-reg; "t_4" = CD4+ naive thymocytes; "t_8" = CD8+ naive thymocytes; "g8a" = small intestinal CD8aa+ intraepithelial lymphocytes; "s_r" = splenic T-reg; "s_4" = splenic CD4+ naive T cells and "s_8" = splenic CD8+ naive T cells); column 16 is identical to column 15 except that column 16 shows whether the sample of origin was a CD25+ or CD25– fraction of a thymic or splenic T-reg population.

本数据集文件包含通过多重PCR扩增cDNA分子后经Illumina测序获取的小鼠T细胞受体（T cell receptor, TCR）序列。所有序列均通过MIGEC软件比对至小鼠基因组，详细信息参见doi: 10.1038/nmeth.2960。除表头行外，每一行对应一条唯一的TCR核苷酸序列信息。第1至11列为MIGEC软件的输出结果。第12至16列为样本来源相关信息，详细说明如下：第12列"sample_id"为样本来源的标识符；第13列"tcr_b"用于标注供体小鼠的TCRβ链状态，其中"poly"代表C57BL/6近交系小鼠；第14列"organ"用于标注样本来源器官，其中"thymus"代表胸腺，"gut"代表小肠与脾脏；第15列"subset"用于标注来源T细胞亚群，各标识含义如下："t_p"代表分选前胸腺细胞；"t_1"代表第一波胸腺细胞；"t_r"代表胸腺调节性T细胞；"t_4"代表CD4+初始胸腺细胞；"t_8"代表CD8+初始胸腺细胞；"g8a"代表小肠CD8aa+上皮内淋巴细胞；"s_r"代表脾脏调节性T细胞；"s_4"代表脾脏CD4+初始T细胞；"s_8"代表脾脏CD8+初始T细胞；第16列与第15列内容基本一致，仅用于标注样本来源是否为胸腺或脾脏调节性T细胞群体中的CD25+或CD25–亚组分。