Single cell sequencing of human mesenchymal stem/stromal cells (MSCs) upon inflammatory licensing

We report changes of the transcriptome profile at the level of single MSCs in response to inflammatory trigger. This process is known as "MSC licensing" and represents a physiological counterregulatory mechanism. MSCs are a heterogenous population, which is highlighted by the according clustering. Overall design: Bone marrow-derived MSCs from healthy donors were cultured for 24 h in presence/absence of IFN-? (10 ng/ml) and TNF-a (15 ng/ml). Subsequently, cells were subjected to droplet single-cell RNA-sequencing.