VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) FOR BETTER WOUND HEALING THROUGH ENHANCED ANGIOGENESIS
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present invention discloses and claims a functionally active peptide of VEGF of SEQUENCE ID NO.: 3 through primers for RT-PCR for cloning the c-DNA obtained from VEGF， comprising a forward primer of 28 bp under SEQUENCE ID NO.: 2 and the reverse primer under SEQUENCE ID NO.: 1 of 32 bp. In addition， the present invention discloses a process of preparation of VEGF comprising the steps of isolating the RNA of VEGF (SEQUENCE ID NO.: 3); RT-PCR of VEGF using primers of SEQUENCE ID NO.: 1 and 2， and collecting the purified c DNA; ligating the clones as obtained with the vector pTZ57R/T; preparing the competent cells; transforming the cloned cells after selecting through antibiotic resistance in vector; isolating the protein obtained; and purifying said obtained VEGF protein.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
