Escherichia coli Raw sequence reads

RNAs can begin to fold immediately after emerging from RNA polymerase during transcription. Some RNAs form structural intermediates that can rearrange during transcription into functional forms. These sequencing datasets were generated using cotranscriptional SHAPE-Seq which measures structural information of nascent RNAs during transcription. The datasets describe the in vitro folding pathways of the E. coli signal recognition particle RNA mutants designed to disrupt and rescue its folding pathway. These datasets serve as inputs into computational RNA structure modeling algorithms that can reconstruct models of the folding pathway.