The source file for unprocessed immuno-blots for MOLECULAR-CELL-D-17-01671

In this study, we investigated the role of p300 in mediating a novel protein lysine acylation, lysine 2-hydroxyisobutyration (Khib), as well as its functional impact on cellular glucose metabolism. Using the immuno-blotting method, we show that p300 functions as a lysine 2-hyroxyisobutyryltransferase to modify both histones and glycolytic enzymes, activating gene transcription and promoting glycolysis.

在本项研究中，我们探讨了p300在介导新型蛋白质赖氨酸酰化——赖氨酸2-羟基异丁酰化（Khib）中的作用，及其对细胞葡萄糖代谢的功能影响。通过免疫印迹法，我们证实p300作为赖氨酸2-羟基异丁酰基转移酶，能够修饰组蛋白和糖酵解酶，激活基因转录并促进糖酵解过程。